DNA Diagnostics by Surface-Bound Melt-Curve Reactions

DNA diagnostics by surface-bound melt-curve reactions.

Melting-curve procedures track DNA denaturation in real time and so provide an effective way of assessing sequence variants. Dynamic allele-specific hybridization (DASH) is one such method, based on fluorescence, which uses heat to denature a single allele-specific probe away from one strand of a polymerase chain reaction product attached to a solid support. DASH is a proven system for research...

Title : Quantitation of DNA methylation by melt curve analysis

In the submitted manuscript entitled “Quantitation of methylation by melt curve analysis”, the authors provided a novel method to quantitate DNA methylation using the raw fluorescence melt data obtainable following PCR amplification of bisulfite modified DNA. The development of a rapid, simple, low-cost, convenient and reliable method to quauntify methylation is certainly important. However, ba...

Bound Lac repressor protein differentially inhibits the unwinding reactions catalyzed by DNA helicases.

A partial duplex DNA substrate containing the Lac repressor binding site, within the duplex region, was constructed to examine the effect of bound Lac repressor on the unwinding reaction catalyzed by several DNA helicases. The substrate contained 90 base pairs of double-stranded DNA and, in the absence of Lac repressor, was effectively unwound by each of the seven helicases tested. The unwindin...

Growth Curve Models and Statistical Diagnostics

Microbial Typing by Machine Learned DNA Melt Signatures

There is still an ongoing demand for a simple broad-spectrum molecular diagnostic assay for pathogenic bacteria. For this purpose, we developed a single-plex High Resolution Melt (HRM) assay that generates complex melt curves for bacterial identification. Using internal transcribed spacer (ITS) region as the phylogenetic marker for HRM, we observed complex melt curve signatures as compared to 1...